种属 |
小鼠 |
别称 |
HL1 |
组织来源 |
小鼠心肌细胞 |
疾病 |
转化细胞系 |
传代比例/细胞消化 |
1 :2传代 ,消化2-3分钟 |
完全培养基配置 |
Claycomb Basal Medium培养基;10%胎牛血清 ;2mM Glutamax;1%Anti-Anti;100 μM Norepinephrine; |
简介 |
HL-1 Cardiac Muscle Cell Line is an immortalized mouse cardiomyocyte cell line able to continuously divide and spontaneously contract while maintaining a differentiated cardiac phenotype. HL-1 can be serially passaged without losing its differentiated cardiac myocyte phenotype, including morphological, biochemical, and electrophysiological properties. HL-1 cells have been used to study normal cardiomyocyte function with regard to signaling, electrical, metabolic, and transcriptional regulation as well as to address pathological conditions such as hypoxia, hyperglycemia-hyperinsulinemia, apoptosis, and ischemia-reperfusion. |
形态 |
心肌细胞样 |
生长特征 |
贴壁生长 |
倍增时间 |
每周 2 至 3 次 |
培养条件 |
气相 :空气 ,95% ;二氧化碳 ,5%。 温度 :37摄氏度 ,培养箱湿度为70%-80%。 |
冻存条件 |
冻存液 :90%FBS ,DMSO 10%, 或使用非程序冻存液 :官网货号JY-H040 |
保藏机构 |
Millipore;SCC065 |
备注 |
该细胞贴壁性不牢,需使用Corning的cellbind细胞培养瓶,货号是3289 ,或者用包被液包被的培养板应放在培养箱(37℃)下至24h以上,或者提前一天。 |
产品使用 |
仅限于科学研究 ,不可作为动物或人类疾病的治疗产品使用。 |